Chun-Ping Jen received the B.S. and Ph.D. degrees from the National Tsing Hua University, Hsinchu, Taiwan, in 1995 and 2000, respectively. He did the Postdoctoral Research with National Cheng Kung University, Tainan, Taiwan (2001–2004) and was a Visiting Scholar and an Assistant Research Scientist from 2004 to 2006 with Center for Applied Nanobioscience, Arizona State University, Phoenix, AZ, USA. He is currently a Full Professor with the Department of Mechanical Engineering, National Chung Cheng University, Chiayi, Taiwan. He has authored or coauthored more than 100 international journal and conference papers. He is working on development of micro/nanodevices of BioMEMS modules and multiphysical simulation.

With extensive experience in researches on modification and application of magnetic beads as well as related knowledge, we have successfully modified aptamer on gold surface effectively. Thiol-PEG-carboxylic bridge has been used by the thiol-gold interaction, which allows the formation of the carboxyl functional groups on the gold substrate (2017-2018). Next, the EDC/NHS solution was used to activate the carboxyl groups for crosslinking to a DNA aptamer. Due to the specific binding between the aptamer and the target cells, we successfully captured the targets and then detected them by the electrochemical impedance spectroscopy (EIS) method. A subsequent study of ours was also performed based on magnetic beads for isolation of target cells (2019-2020). In this study, we modified aptamers onto the surface of carboxylated-magnetic beads via carbodiimide crosslinker and specific aptamers. Using the magnetic properties induced by immunomagnetic beads attached to the target cells, the immunomagnetic beads-conjugated cells can be effectively separated and isolated. In our other study, gold nanoparticles were also successfully developed and synthesized with the desired sizes based on the Natan method, after that, the penetration of these gold nanoparticles into cells was also conducted by the application of electroporation method. Basing on some of the above studies, a process of synthesis and chemical modification to gold nanoparticles for conjugation to target cells will be performed. At the same time, the penetration of gold nanoparticles to cells will also be investigated and compared based on electroporation and cold atmospheric plasma (CAP) treatment.