Research and development with 14 years of experience in the functional study of globo-series glycosphingolipids and cancers. Adept in handling several research projects that advanced in the interaction of biological mechanisms and glycans. Can accomplish research design, experimental data analytics, investigation, and development in a wide range of therapeutic areas. Looking to leverage well collaboration, research planning, and new technique contribution skill in studying the interplay of glycosphingolipids and antitumor immunity.

I would like to bring the novel knowledge of glycobiology, oncology, and immune therapy back to Taiwan, especially the correlation between cellular biochemistry and glycoscience. In my current research, we found that the tumor cells not only need highly glycosyl metabolism pathways for survival but also express specific glycans for promoting cancer survival signals and escaping immune cell attacks (as known as immunosuppression). 

On the other hand, We are making an effort to enhance antibodies' effectivity and antibody-vaccinal effects by glycosyl-engineering on the Fc domain in the animal study. This modification made that human immune cells quickly recognized to antibodies and increased binding affinity for ADCC(Antibody-dependent cellular cytotoxicity) or ADCP(Antibody-dependent cellular phagocytosis).

In our Lab, you will learn the below devices that would be as a tool for addressing our research and getting the skill to work in the bio-industry.

1. Flow cytometry: To analyze the cell surface marker and sort out specific cell populations for further cell functional assay or interactions.
2. LC-MS/MS: Profiling the cell composition and molecular and glycans sequencing.
3. Laminar flow: For cell culturing and cell assay.
4. Antibody glycoengineering.
5. Animal study for tumor therapy.
6. Colony technique: You will get the skill to edit the gene sequence and build up your own plasmid to transform this plasmid in an expression host (ex: E.coli)
7. Protein expression and purification: You can transfect target plasmid in the mammalian cells to express target protein and purification pure protein form mixture as well.
8. Confocal Microscope: To grab images from living cells or fixation cells by Fluorescence labeling.
9. Immune cell isolation and culturing: To isolate specific immune cells as cell sources for cell therapy, including NK cells and Macrophages.
10. Glycan array and lectin array: For screening the interacted glycans.
11. Biacore (Surface. Plasmon resonance, SPR): A label-free kinetic affinity analysis for protein-protein interaction and protein-glycan interaction.
12. Western blot and qPCR for verification of protein and gene expression.