National Chung Hsing University

Bio-Organic Material Laboratory

Cheng-Chung Chang
https://sites.google.com/site/ccchanggroup/home
Introduction

Developments of targeting fluorophores, biosensor photosensitizers, and NIR biomarkers, photodynamic therapy, nanomaterials, optical study of spectra; especially the SERS (Surface-enhanced Raman spectroscopy)

Dr. Cheng-Chung Chang completed post-doctoral studies at IAMS, Academia Sinica of Taiwan in development for biosensors and drug discovery in 2006. He is currently a Distinguished Professor of Graduate Institute of Biomedical Engineering, National Chung Hsing University of Taiwan. Recently, Professor Chang focused the research of Raman spectroscopy applied to biomedical detection. He invented some novel nanochips and applied them to SERS (Surface-enhanced Raman spectroscopy) and established a digital authentication barcode recognition system to successfully used it on biomedical (bilirubin, cancer cell , virus, DNA, antibody and antigen, and COVID-19) and agricultural (pesticides, orchid virus) detections. At the same time, Professor Chang's team has also successfully used their system to detect the covid-19 virus, and can even track the antibodies produced in the human body after virus infection or vaccination in human blood. These achievements contribute to the well-being of human health.

Our laboratory consists of graduate students with chemical backgrounds and biomedical fields, and postdoctoral fellows focusing on organic fluorescent molecules synthesis, Surface-Enhanced Raman Spectroscopy, and Biological experiments. 

We are dedicated to developing the technology of detection and targeting that can be applied in clinical and actual fields. Finally, cooperate with hospitals to develop more effective, accurate, and safer biomedical products by combining clinical treatment and detection.

Research Topics

.Our laboratory has been dedicated to the research of photodynamic therapy (PDT) materials for many years, including the design and fabrication of PDT drugs. Our research focuses on various types of photosensitizers, evaluating their toxicity, singlet oxygen generation ability, fluorescence resonance energy transfer (FRET) efficiency, and specificity towards cancer cells. We have designed a series of binary complex compounds by covalently bonding BMVC to Porphyrin, and identified the optimal covalent bonding ratio. Among them, the 2O-M(H2) molecule formed by the ortho-position bonding of BMVC to Porphyrin exhibits the best therapeutic effects in PDT. This molecule possesses the following characteristics: (1) Specific recognition of cancer cells and provides multiple selectable irradiation wavelengths; (2) Shows noticeable color changes in cell imaging, enabling us to track the molecular transport mechanism and serve as a cell death marker. Based on the experience gained from the structure-activity relationship (SAR) studies, we provide a blueprint for the design of the next generation of PDT photosensitizers. We have improved FRET-PDT to achieve more significant selective phototherapy effects.

In addition, our laboratory has developed a series of self-made photosensitizing materials based on BODIPY. BODIPY itself possesses excellent optical properties, and through our modifications, we have discovered its unique photosensitive characteristics, which is a significant finding. We have also found that the modified precursor substance of methylene blue (MB), phenothiazine, can be developed into a PDT material for infrared light. Furthermore, we have discovered a novel type of ROS, type I, photosensitizing substance that differs from singlet oxygen production mechanisms. In summary, these photosensitizing materials possess the following features:

  1. Small-molecule fabrication, increasing cellular uptake.
  2. Selective toxicity towards cancer cells while leaving normal cells unharmed.
  3. Very low dark toxicity.
  4. Excellent photostability.
  5. Fluorescent properties.
  6. Retention of biosensor characteristics, enabling differentiation between normal cells and cancer cells, or between live and dead cells.

All the organic molecules used in this project are synthesized or modified in our laboratory. A substantial portion of our work involves testing the conditions for molecule synthesis, ROS spectral measurement, and identification, and only when expected products are obtained, we proceed with cell experiments and biological applications.

Similarly, we have explored a novel non-invasive tumor treatment approach based on photodynamic therapy, known as Sonodynamic Therapy (SDT). SDT is a non-invasive treatment method for various cancers, offering greater tissue penetration depth compared to photodynamic therapy (PDT). Similar to clinical PDT, SDT utilizes low-intensity focused ultrasound to stimulate sonosensitizers (SS) and generate toxic reactive oxygen species (ROS) that specifically target tumor cells. We have synthesized a range of derivative molecules, including Xanthene, Methylene, BODIPY, Phenothiazine, and Acridine, as sonosensitizers. Through spectroscopic analysis, we have identified optimal ROS generation conditions and applied them in cell toxicity assessments. The significant discovery in SDT is that ultrasound provides the energy required for ROS production. We have developed numerous series of derivatives that can serve as sonosensitizers for killing cancer cells.

Furthermore, our laboratory utilizes Raman spectroscopy for analysis and detection. However, the weak Raman signal and susceptibility to fluorescence interference have long posed challenges in detection. To overcome these issues, we have developed a comprehensive Surface-Enhanced Raman Spectroscopy (SERS) detection platform called the Comprehensive SERS Detection Platform (CSDP) system. The CSDP system consists of two components: the 3D-PHS (3-dimensional plasmonic hotspots) nanochip and signal processing software. The 3D-PHS nanochip is based on a randomly intersected silver nanowire (AgNW) scaffold, which induces localized surface plasmon resonance (LSPR) and a uniform distribution of hotspots, forming a unique three-dimensional plasmonic-enhanced nanochip. This nanochip enhances the Raman signals of target molecules by over 1000-fold sensitivity. The advantages of 3D-PHS include no need for sample pretreatment or sample destruction, minimal sample volume (20 μL), short detection time (less than 5 minutes), antibody-free biomedical detection, long chip stability (over 80 days), and compatibility with any Raman spectrometer. Our team has also developed signal processing software with functions such as signal identification, optimization of imperfect or uneven data, particularly suitable for clinical and on-site detection. The software converts the identified Raman spectra into a database, transforming molecular fingerprints into barcode (BAR-CODE) format for convenient data processing and encoding. By integrating the 3D-PHS nanochip and signal processing software into the CSDP system, it can be applied to any Raman spectrometer, especially our developed portable spectrometer, making CSDP a powerful rapid screening and detection system. CSDP has been successfully applied in fields such as agriculture (pesticides, orchid viruses) and biomedical detection (drugs, bilirubin, bacteria, cancer cells, antibodies, antigens, and coronaviruses). The pesticide detection has been successfully implemented, and the bilirubin detection in collaboration with National Taiwan University Hospital has entered the stage of clinical data collection. Data for Covid-19 viruses and antibodies has also been established. This bioprobe technology can be effectively used for point-of-care testing (POCT) in healthcare settings. The 3D-PHS nanochip and CSDP system developed by our team possess high sensitivity, specificity, ease of preparation, and rapid detection, with the advantages of not requiring antibodies or additional sample purification and preparation steps. These characteristics make it a powerful detection platform with broad application prospects.

Honor
  1. Sahoo, S. R.; Huey-Jen Hsu, S.; Chou, D. A.; Wang, G. J.; Chang, C. C. Biosens and Bioelectron 2022, 213, 114440 (SCI, IF =12.545 )
  2. Zi-Lun Lai, Jui-Shan Chang, Yung-Chieh Chan, Cheng-Chung Chang,* Chia-Ying Li, Shih-Wei Huang.* Tumor tissues diagnosis with PIEE lipid droplet vesicles. Sensors and Actuators B: Chemical 2021, 331,129269 (SCI, IF =8.42 )
  3. Manik Chandra Sil, Li-Syuan Chen, Chin-Wei Lai, Yu-Hsin Lee, Cheng-Chung Chang, * and Chih-Ming Chen,* Enhancement of power conversion efficiency of dye-sensitized solar cells for indoor applications by using a highly responsive organic dye and tailoring the thickness of photoactive layer. Journal of Power Sources 2020, 479, 229095(SCI, IF= 9.794)
  4. Manik Chandra Sil, Li-Syuan Chen, Chin-Wei Lai, Cheng-Chung Chang,* and Chih-Ming Chen,* 2020, “Enhancement of Solar Efficiency of Dye-Sensitized Solar Cell by Molecular Engineering of Organic Dye Incorporating N-alkyl Attached 1, 8-Naphthalamide Derivative”, Journal of Materials Chemistry C 2020,8, 11407-11416. (Selected as “Back Cover”) (SCI, IF= 8.067 )
  5. Zi-Jun Liu , Shu-Ching Lin , Pei-Yuan Lee , Ying-Ting Lin , Zi-Lun Lai , Cheng-Chung Chang* and Gou-Jen Wang* Dual-acting antibacterial porous chitosan film embedded with a photosensitizer, Science and Technology of Advanced Materials. 2020, 21:1, 562-572. (SCI, IF= 8.09)
  6. Chien CM, Wu PC, Satange R, Chang CC, Lai ZL, Hagler LD, Zimmerman SC,* Hou MH.* Structural Basis for Targeting T:T Mismatch with Triaminotriazine-Acridine Conjugate Induces a U-Shaped Head-to-Head Four-Way Junction in CTG Repeat DNA. J Am Chem Soc. 2020;142(25):11165-11172. (SCI, IF= 15.04)
  7. Wu, H.-J.; Chang, C.-C.* Fabrication of Double Emission Enhancement Fluorescent Nanoparticles with Combined PET and AIEE Effects. Molecules 2020, 25, 5732. (SCI, IF= 4.927)
  8. Chan, Y.-C.; Li, C.-Y.; Lai, C.-W.; Wu, M.-W.; Tseng, H.-J.; Chang, C.-C.* Synthesis and Application in Cell Imaging of Acridone Derivatives. Appl. Sci. 2020, 10, 8708. (SCI, IF= 3.021)
  9. Chien-Hui Su, Jyun-Wei Chen, Li-Da Chen, Jui-Chih Chang, Chin-San Liu, Cheng-Chung Chang * and Gou-Jen Wang, Organic small molecule for detection and photodegradation of mitochondrial DNA mutations. J. Mater. Chem. B, 2019, 7, 5974-5955. (SCI, IF= 7.571)
  10. Chang-Yi Peng, Che-Wei Hsu, Ching-Wen Li, Po-Lin Wang, Chien-Chung Jeng, Cheng-Chung Chang,* and Gou-Jen Wang*, Flexible Photonic Crystal Material for Multiple Anticounterfeiting Applications. ACS Appl. Mater. Interfaces 2018, 10, 9858−9864. (SCI, IF= 9.44)
  11. Ying-Chen Chiang, Zi-Lun Lai, Chih-Ming Chen, Cheng-Chung Chang* and Bin Liu.* Construction of emission-tunable nanoparticles based on a TICT-AIEgen: impact of aggregationinduced emission versus twisted intramolecular charge transfer. J. Mater. Chem. B, 2018,6, 2869-2876. (SCI, IF= 7.571)
  12. Ting-Yuan Tseng, Wei-Wen Chen, I-Te Chu, Chiung-Lin Wang, Cheng-Chung Chang, Mei-Chun Lin, Pei-Jen Lou and Ta-Chau Chang.* The G-quadruplex fluorescent probe 3,6-bis(1-methyl-2-vinylpyridinium) carbazole diiodide as a biosensor for human cancers. Scientific Reports,2018,8:16082, DOI:10.1038/s41598-018-34378-8. (SCI, IF=4.996)
  13. Pin-Hui Li, Li-Heng Liu, Cheng-Chung Chang, Rong Gao, Chung-Hang Leung, Dik-Lung Ma and Hui-Min David Wang. Silencing Stem Cell Factor Gene in Fibroblasts to Regulate Paracrine Factor Productions and Enhance c-Kit Expression in Melanocytes on Melanogenesis. Int J Mol Sci. 2018, 19(5):1475. doi: 10.3390/ijms19051475.(SCI, IF=6.208)
  14. Yu-Jie Lin, Jyun-Wei Chen, Po-Tsung Hsiao, Yung-Liang Tung, Cheng-Chung Chang * and Chih-Ming Chen*. Efficiency improvement of dye-sensitized solar cells by in situ fluorescence resonance energy transfer. J. Mater. Chem. A, 2017, 5, 9081-9089. (SCI, IF=14.511)
  15. Jyun-Wei Chen, Chih-Ming Chen,* Cheng-Chung Chang, * A fluorescent pH probe for acidic organelles in living cells. Org. Biomol. Chem., 2017, 15, 7936–7943. (SCI, IF=3.890)
  16. Ying-Chen Chiang, Chun-Ta Huang, Wei Hsin Wang, Cheng-Chung Chang,* A dual photoluminescence enhancement system: stabilization of a water soluble AIEE fluorogen using silver nanowire. Faraday Discuss., 2017, 196, 55-69. (SCI, IF=4.394)
  17. Yen-Ting Tung, Cheng-Chung Chang, Jyh-Cherng Jud,* and Gou-Jen Wang,* Fabrication of a reticular poly(lactide-co-glycolide) cylindrical scaffold for the in vitro development of microvascular networks. Sci. Technol. Adv. Mater. 2017, 18, 165-185.(SCI, IF=8.09)
  18. Jyun-Wei Chen and Cheng-Chung Chang.* A Dual Anticancer Efficacy Molecule: A Selective Dark Cytotoxicity Photosensitizer. ACS Appl. Mater. Interfaces 2016, 8(44), 29883−29892. (SCI, IF= 9.44)
  19. Yen-Ting Tung, Cheng-Chung Chang,* Yi-Ling Lin, Shie-Liang Hsieh, Gou-Jen Wang,* Development of double-generation gold nanoparticle chip-based dengue virus detection system combining fluorescence turn-on probes. Biosensors and Bioelectronics 2016, 77, 90–98 (SCI, IF=12.545)
  20. Yi-Wen Wang, Ting-Yang Chen, Tsung-Han Yang, Cheng-Chung Chang, Tsung-Lin Yang,* Yu-Hwa Lo,* Jian-Jang Huang.* Thin-Film Transistor-Based Biosensors for Determining Stoichiometry of Biochemical Reactions. PLoS One. 2016, Dec 29; 11(12):e0169094. (SCI, IF=3.752)
  21. Yu-Jie Lin, Cheng-Chung Chang,* Sheng-Jye Cherng, Jyun-Wei Chen and Chih-Ming Chen** Manipulation of light harvesting for efficient dye sensitized solar cell by doping an ultraviolet light-capturing fluorophore. Prog. Photovolt: Res. Appl. 2015, 23(1), 106–111. (SCI, IF=8.05)
  22. Ta-Chau Chang*, Zi-FuWang, Ting-Yuan Tseng, Cheng-Chung Chang. Novel molecules targeting DNA G-quadruplexes: carbazole derivatives. Biological Relevance & Therapeutic Applications of DNA- & RNA-Quadruplexes, CHAPTER 13. July 2015, Pages 192-204.
  23. Ting-Yuan Tseng, Cheng-Chung Chang, Jing-Jer Lin and Ta-Chau Chang*. A Fluorescent Anti-Cancer Agent, 3,6-bis(1-methyl-4-vinylpyridinium) Carbazole Diiodide, Stains G-Quadruplexes in Cells and Inhibits Tumor Growth. Current topics in medicinal chemistry 2015, 15, 1964-70. (SCI, IF=2.9).
  24. Wei-Chun Huang, Ting-Yuan Tseng, Ying-Ting Chen, Cheng-Chung Chang, Zi-Fu Wang, Chiung-Lin Wang, Tsu-Ning Hsu, Pei-Tzu Li, Chin-Tin Chen, Jing-Jer Lin, Pei-Jen Lou and Ta-Chau Chang*. Nucleic Acids Research 2015, 43(21): 10102–10113 (SCI, IF=19.33)
  25. Tung-Sheng Hsieh, Jhen-Yi Wu and Cheng-Chung Chang*, Multiple fluorescent behaviors of phenothiazine-based organic molecules, Dyes and Pigments 2015, 112, 34-41. (SCI, IF=5.122) NSC 101-2113-M-005-016-MY3
  26. Tung-Sheng Hsieh, Jhen-Yi Wu and Cheng-Chung Chang*. Synthesis of a Photostable Near-Infrared-Absorbing Photosensitizer for Selective Photodamage to Cancer Cells. Chemistry - A European Journal 2014, 20, 9709 – 9715. (SCI, IF=5.771) NSC 101-2113M-005-016-MY3
  27. Yen-Chih Lai and Cheng-Chung Chang.* Photostable BODIPY-based molecule with simultaneous type I and type II photosensitization for selective photodynamic cancer therapy. Journal of Materials Chemistry B 2014, 2(11), 1576-1583. (SCI, IF=7.571) NSC 101-2113-M-005-016-MY3
  28. Cheng-Chung Chang.* Can biologically inspired patches yield a new age of transdermal delivery? Therapeutic Delivery, Editorial, 2014, 5(4), 373-375. NSC 101-2113M-005-016-MY3
  29. Yung-Chieh Chan, Jyun-Wei Chen, Sheng- Yuan Su and Cheng-Chung Chang.* Direct visualization of the quadruplex structures in human chromosome using FRET: Application of quadruplex stabilizer and duplex-binding fluorophore. Biosensors and Bioelectronics 2013, 47(15), 566–573. (SCI, IF= 12.545)
  30. Yen-Chih Lai, Sheng-Yuan Su and Cheng- Chung Chang.* Special Reactive Oxygen Species Generation by a Highly Photostable BODIPY-Based Photosensitizer for Selective Photodynamic Therapy. ACS Appl. Mater. Interfaces 2013, 5(24), 12935−12943. (SCI, IF=9.44)
  31. Chen-Yi Tsai and Cheng-Chung Chang.* Auto-adhesive transdermal drug delivery patches using beetle inspired micropillar structures. Journal of Materials Chemistry B 2013, 1(43), 5963-5970. (SCI, IF=7.571) NSC 101-2113-M-005-016-MY3
  32. Yung-Chieh Chan, Jyun-Wei Chen, Sheng- Yuan Su, Cheng-Chung Chang.* Aggregation induced photodynamic therapy enhancement based on linear and nonlinear excited FRET of fluorescent organic nanoparticles. Journal of Materials Chemistry B 2013, 1(18), 2350-2357. (SCI, IF=7.571) NSC 101-2113-M-005-016-MY3
  33. Chi-Chih Kang, Wei-Chun Huang, Chiung-Wen Kouh, Zi-Fu Wang, Chih- Chien Cho, Cheng- Chung Chang, Chiung-Lin Wang, Ta-Chau Chang,* Joachim Seemann and Lily Jun-shen Huang. Chemical principles for the design of a novel fluorescent probe with high cancer-targeting selectivity and sensitivity. Integrative Biology 2013, 5(10), 1217-1228. (SCI, IF=3.371)
  34. Ting-Yuan Tseng, Cheng- Hao Chien, Jen- Fei Chu, Wei- Chun Huang, Mei-Ying Lin, Cheng-Chung Chang, Ta-Chau Chang.* Fluorescent probe for visualizing guanine-quadruplex DNA by fluorescence lifetime imaging microscopy. Journal of Biomedical Optics 2013, 18(10), 101309-1-6. (SCI, IF= 3.758)
  35. Fong-Chun Huang, Cheng-Chung Chang, Jing-Min Wang,Ta-Chau Chang and Jing-Jer Lin1*, “Induction of senescence in cancer cells by the G-quadruplex stabilizer, BMVC4, is independent of its telomerase inhibitory activity”, British Journal of Pharmacology, 2012, 167(2), 393–406.
  36. Ya-Shuan Chou, Cheng-Chung Chang, Ta-Chau Chang, Tsung-Lin Yang, Tai-Horng Young, Pei-Jen Lou*. Photo-induced antitumor effect of 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide. BioMed Research International, 2013, 930281. (SCI, IF=3.411)
  37. Cheng-Chung Chang,* Meng-Chieh Hsieh, Jung-Chih Lin, Ta-Chau Chang. Selective photodynamic therapy based on aggregation-induced emission enhancement of fluorescent organic nanoparticles. Biomaterials 2012, 33(3), 897-906. (SCI, IF= 15.863)
  38. Wang-Hua Hong, Chu-Chieh Lin, Tung-Sheng Hsieh, Cheng-Chung Chang.* Preparation of fluoroionophores based on diamine-salicylaldehyde derivatives. Dyes and Pigments 2012, 94, 371–379. (SCI, IF=5.122) NSC 99-2113-M-005-011-MY2
  39. Hsin-Hung Lin, Yung-Chieh Chan, Jyun-Wei Chen, Cheng-Chung Chang.* Aggregation- induced emission enhancement characteristics of naphthalimide derivatives and their applications in cell imaging. Journal of Materials Chemistry 2011, 21, 3170-3177. (SCI, IF=6.7.571) NSC 99-2113-M-005-011-MY2
  40. Sheng-Yuan Su, Hsin-Hung Lin, Cheng-Chung Chang.* Dual optical responses of phenothiazine derivatives: near-IR chromophore and water-soluble fluorescent organic nanoparticles. Journal of Materials Chemistry 2010, 20, 8653–8658. (IF=7.571)
  41. Cheng-Liang Peng, Ping-Shan Lai, Cheng-Chung Chang, Pei-Jen Lou, Ming-Jium Shieh.* The synthesis and photodynamic properties of meso-substituted, cationic porphyrin derivatives in HeLa cells. Dyes and Pigments 2010. 84, 140-147. (IF=5.122)
  42. Hsin-Hung Lin, Sheng-Yuan Su, Cheng-Chung Chang * “Fluorescent Organic Nanoparticles Formation in Lysosomes for Cancer Cell Recognition”, ORGANIC & BIOMOLECULAR CHEMISTRY ,2009, 7, 2036–2039.
  43. Hsin-Hung Lin; Cheng-Chung Chang*, ”Spectroscopic Investigations of Vinyl-Substituted 10H-Phenothiazine”, Dyes and Pigments., 2009, 83 230–236.
  44. Chi-Chih Kang, Chin-Tin, Chen, Chih-Chien Cho, Yu-Chen Lin, Cheng-Chung Chang,* Ta-Chau Chang*, "A Dual Selective Antitumor Agent and Fluorescence Probe: the Binary BMVC–Porphyrin Photosensitizer", Chem Med Chem, 3(5) , 725-728. (2008)
  45. Fong-Chun Huang Cheng- Chung Chang, Pei-Jen Lou, I-Chun Kuo, Chih-Wei Chien,Chin-Tin Chen, Fu-Ying Shieh,Ta-Chau Chang*, and Jing-Jer Lin*, "G-Quadruplex Stabilizer 3,6-Bis(1-Methyl-4-Vinylpyridinium) Carbazole Diiodide Induces Accelerated Senescence and Inhibits Tumorigenic Properties in Cancer Cells," Mol Cancer Res, 6(6) 955-964. (2008)
  46. Cheng-Chung Chang Yu-Tsai Yang, Jen-Chang Yang, Hong-Da Wu, Tsuimin Tsai*, "Absorption and emission spectral shifts of rose bengal associated with DMPC liposomes," Dyes and Pigments, 79 170-175. (2008)
  47. Cheng-Liang Peng, Ming-Jium Shieh* , Ming-Hsien Tsai, Cheng-Chung Chang, Ping-Shan Lai*, "Self-assembled star-shaped chlorin-core poly(3-caprolactone)–poly(ethylene glycol) diblock copolymer micelles for dual chemo-photodynamic therapies," Biomaterials, 29 ,3599-3608. (2008)
  48. Cheng-Chung Chang , Chih-Wei Chien, Yi-Hsueh Lin and Ta-Chau Chang*, "Investigation of spectral conversion of d(TTAGGG)4 and d(TTAGGG)13 upon potassium titration by a G-quadruplex recognizer BMVC molecule," Nucleic Acids Research, 35(9), 2846-2860. (2007)
  49. Chi-Chih Kang, Cheng-Chung Chang, Ta-Chau Chang*, Li-Jen Liao, Pei-Jen Lou, Wenjun Xie and Edward S. Yeung, "A handheld device for potential point-of-care screening of cancer," The Analyst, 132, 745-749. (2007)
  50. Yu-Lin Tsai, Cheng-Chung Chang, Chi-Chih Kang and Ta-Chau Chang*, "Effect of different electronic properties on 9-aryl-substituted BMVC derivatives for fluorescence probes," Journal of Luminescence, 127, 41-47. (2007)
Educational Background

EDUCATION

  • 1993-1999 Ph.D., Department of Chemistry, Tamkang University
  • 1991-1993 MS, Department of Chemistry, Tamkang University
  • 1987-1991 B.S., Department of Chemistry, Tamkang University

Current Position:

  • Associate Dean, College of Engineering, National Chung Hsing University
  • Distinguished Professor, Institute of Biomedical Engineering, National Chung Hsing University

Experience:

  • Director, Engineering Center, College of Engineering, National Chung Hsing University (2021/01-present)
  • Director, Center for Smart Minimally Invasive Instruments, National Chung Hsing University (2018/01-present)
  • Director, Institute of Biomedical Engineering, National Chung Hsing University (2014/08-2018/07)
  • Distinguished Professor, Institute of Biomedical Engineering, National Chung Hsing University (2018/08-present)
  • Professor, Institute of Biomedical Engineering, National Chung Hsing University (2014/08-2018/07)
  • Associate Professor, Institute of Biomedical Engineering, National Chung Hsing University (2011/08-2014/07)
  • Assistant Professor, Institute of Biomedical Engineering, National Chung Hsing University (2009/08-2011/07)
  • Assistant Professor, Department of Chemistry, National Chung Hsing University (2006/08-2009/07)