National Yang Ming Chiao Tung University

Bacteriology Lab

Cheng-Yen Kao
https://sites.google.com/view/kaolab/

Research Field

Medicine

Introduction

  • 2024/05 ~ Part-time Consultant, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. 
  • 2024/02 ~ Associate Professor, Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taiwan.
  • 2024/02 ~ Jointly Appointed Associate Professor, Biomedical Industry Ph.D program, National Yang Ming Chiao Tung University, Taiwan.
  • 2024/02 ~ Jointly Appointed Associate Professor, Program in Molecular Medicine, National Yang Ming Chiao Tung University, Taiwan. 
  • 2023/08 ~ 2025 Deputy Director, Microbiota Research Center, College of Medicine, National Yang Ming Chiao Tung University, Taiwan. 
  • 2021/02 ~ 2024/01 Jointly Appointed Assistant Professor, Biomedical Industry Ph.D program, National Yang Ming Chiao Tung University, Taiwan.
  • 2020/08 ~ 2024/01 Jointly Appointed Assistant Professor, Program in Molecular Medicine, National Yang Ming Chiao Tung University, Taiwan. 
  • 2019/08 ~ 2024/01 Assistant Professor, Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taiwan.
  • 2017/10 ~ 2019/07 Postdoctoral fellow, Department of Medical Microbiology and Immunology, University of Wisconsin-Madison (Dr. John-Demian (JD) Sauer Lab).
  • 2016/08 ~ 2017/10 Postdoctoral fellow, Department of Biotechnology and Laboratory Science in Medicine, School of Biomedical Science and Engineering, National Yang Ming University, Taipei, Taiwan (Prof. Jiunn-Jong Wu Lab).
  • 2014/08 ~ 2016/07 Postdoctoral fellow, Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan (Prof. Jiunn-Jong Wu Lab).

The majority of our work has been centered on Helicobacter pylori, a bacterium that infects 50% of the global population and is linked to the development of gastric cancer. Key factors such as H. pylori adhesins and flagella-based motility are crucial for establishing infection, so my research has focused on regulating these adhesin and flagella-related genes. Our current research shifts towards identifying novel genes involved in bacterial metabolism and intracellular survival in Listeria monocytogenes. We have identified the regulon of a novel transcriptional regulator, Lmo1576, and demonstrated its role in regulating metabolism for cytosolic survival in L. monocytogenes. We have also collaborated with various groups and clinical departments to study the epidemiology of antimicrobial resistance in Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella spp., and Acinetobacter baumannii. In addition, through collaboration with Dr. Sauer (University of Wisconsin-Madison), we have identified several small-molecule inhibitors of bacterial PASTA kinases, a conserved family of proteins critical for virulence and antibiotic resistance in pathogens such as L. monocytogenes, Staphylococci, Enterococci, and Mycobacteria. Beyond developing these inhibitors as novel antibiotics, we are also investigating the downstream signaling pathways of PASTA kinases that regulate antibiotic resistance and virulence, with a focus on L. monocytogenes and methicillin-resistant Staphylococcus aureus (MRSA).


Research Topics
  1. Antimicrobial resistance mechanisms; 
  2. Bacterial genomics and NGS;
  3. Virulence factors and bacterial pathogenesis;
  4. Gene regulation and transcriptome;
  5. Mouse model for bacterial infection; 
  6. Antimicrobial-drug and probiotic development.

Honor
  1. 2023. National Yang Ming Chiao Tung University Research Reward Subsidies (2023 Aug to 2025 Jul)
  2. 2020. Outstanding alumni, Department of Biochemical Science and Technology, National Chia-Yi University.
  3. 2019-2021. American Society for Microbiology (ASM) Young Ambassador to Taiwan.
  4. 2018. MMPC Fellowship/Travel Award. 25th Annual Midwest Microbial Pathogenesis Conference (MMPC), University of Iowa, Iowa, IA.

Educational Background
  • 2009/9 ~ 2014/7 Ph.D., Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan.
  • 2005/9 ~ 2007/7 M.S., Institute of Molecular Medicine, National Cheng Kung University, Tainan, Taiwan.