Brain functional imaging

There are four important factors when considering imaging: contrast, resolution, speed, and depth. We work together with world leading brain scientist to develop new technologies to push the boundaries. The aim is to understand how brain works through functional (high-speed) and structural (high-resolution) microscopy. 

S.-H. Huang, N. Irawati, Y.-F. Chien, J.-Y. Lin, Y.-H. Tsai, P.-Y. Wang, L.-A. Chu, M.-L. Li, A.-S. Chiang, K. Tsia, and S.-W. Chu*, “Optical Volumetric Brain Imaging: Speed, Depth, and Resolution Enhancement” J. Phys. D 54, 323002 (2021). Invited Topical Review 

Novel Contrast (SRS, iSCAT, 3PF)

G.-J. Huang, C.-W. Li, P.-Y. Lee, J.-X. Su, K.-C. Chao, L.-A. Chu, A.-S. Chiang, J.-X. Cheng, B.-H. Chen, C.-H. Lu, S.-W. Chu*, and S.-D. Yang,* “Electronic Preresonance Stimulated Raman Scattering Spectromicroscopy Using Multiple-Plate Continuum”, J. Phys. Chem. B 127, 6896-6902 (2023) FutureTech Award 未來科技獎 2023

G.-J. Huang, P.-C. Lai, M.-W. Shen, J.-X. Su, J. Y. Guo, K.-C. Chao, P. Lin, J.-X. Cheng, L.-A. Chu, A.-S. Chiang, B.-H. Chen, C.-H. Lu, S.-W. Chu*, and S.-D. Yang,* “Towards Stimulated Raman Scattering Spectro-Microscopy Across the Entire Raman Active Region Using Multiple-plate Continuum”, Opt. Express 30, 38975-38983 (2022)

Y.-T. Hsiao, T.-Y. Wu, B.-K. Wu, S.-W. Chu, and C.-L. Hsieh*, “Spinning disk interferometric scattering confocal microscopy captures millisecond timescale dynamics of living cells” Opt. Express, 30, 45233-45245 (2022).

K.-J. Hsu, Y.-Y. Lin, A.-S. Chiang, S.-W. Chu*, “Optical properties of adult Drosophila brains in one-, two-, and three-photon microscopy,” Biomed. Opt. Exp. 10, 1627-1637 (2019).

Speed + depth

C.-H. Luo, S. Vyas, K.-Y. Huang, S.-W. Chu, and Y. Luo “Multiplane differential saturated excitation microscopy using varifocal lens” Biomed. Opt. Express 15, 953 (2024)

C.-L. Chung, T. Furieri, J.-Y. Lin, J.-C. Lee, Y.-F. Chen, M.-K. Pan, S. Bonora*, and S.-W. Chu*, ”Plug-and-play adaptive optics for two photon high-speed volumetric imaging,” JPhys Photonics 4, 024003 (2022) invited by special issue of “Focus on Ultrasound-Based Systems for Light Control”, 

Y.-F. Chien, J.-Y. Lin, P.-T. Yeh, K.-J. Hsu, Y.-H. Tsai, S.-K. Chen*, and S.-W. Chu*, “Dual GRIN lens two-photon endoscopy for high-speed volumetric and deep brain imaging” Biomed. Opt. Exp., 12, 162-172 (2021).

C. Huang, C.-Y. Tai, K.-P. Yang, W.-K. Chang, K.-J. Hsu, C.-C. Hsiao, S.-C. Wu, Y.-Y. Lin*, A.-S. Chiang*, and S.-W. Chu*, “All-optical volumetric physiology for connectomics in dense neuronal structures” iScience 22, 133-146 (2019).

K.-J. Hsu, Y.-Y. Lin, Y.-Y. Lin, K. Su, K.-L. Feng, S.-C. Wu, Y.-C. Lin, A.-S. Chiang, S.-W. Chu*, “Millisecond two-photon optical ribbon imaging for small-animal functional connectome study” Opt. Lett. 44, 3190-3193 (2019). Editor’s pick

K.-J. Hsu, K.-Y. Li, Y. Y. Lin, S.-W. Chu, and A.-S. Chiang, “Optimizing depth-of-field extension in optical sectioning microscopy techniques using a fast focus-tunable lens,” Opt. Express 25, 16783-16794 (2017)

Resolution + depth 

Y.-H. Lin, L.-W. Wang, Y.-H. Chen, Y.-C. Chan, S.-H. Hu, S.-Y. Wu, C.-S. Chiang, G.-J. Huang, S.-D. Yang, S.-W. Chu, K.-C. Wang, C.-H. Lin, P.-H. Huang, H.-J. Cheng, B.-C. Chen*, L.-A. Chu*, “Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain” eLife 13, RP93212 (2024) 

H.-Y. Lin, L.-A. Chu, H. Yang, K.-J. Hsu, Y.-Y. Lin, K.-H. Lin, S.-W. Chu*, A.- S. Chiang, “Imaging Through the Whole Brain of Drosophila at λ/20 Super-Resolution,” iScience 14, 164-170 (2019).

I.-C. Su, K.-J. Hsu, P.-T. Shen, Y.-Y. Lin, and S.-W. Chu*, “3D resolution enhancement of deep-tissue imaging based on virtual spatial overlap modulation microscopy,” Opt. Express 24, 16238 (2016)